Biochemistry Quiz# 6 Polymerase Chain Reaction

1. A researcher is using PCR to amplify a specific segment of DNA. During which step of PCR is the DNA template strand separated into two single strands to allow primer binding?

Question 1 of 5

2. Which of the following steps in PCR involves the binding of primers to the single-stranded DNA template? 

Question 2 of 5

3. During which step of PCR does the DNA polymerase synthesize a new strand of DNA by adding nucleotides complementary to the template strand? 

Question 3 of 5

4. In the context of PCR, which enzyme is responsible for synthesizing the new strand of DNA from the dNTPs during the elongation step? 

Question 4 of 5

5. A student is setting up a PCR reaction. Which of the following components is NOT necessary to include in the PCR mixture for DNA amplification? 

Question 5 of 5


 

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